Media at the Touch of a Button
Krakatoa facilities fast iteration leading to optimization of cell culture media conveniently and sustainably. Get yours today at Nucleus Biologics.
Define run specifications and ensure proper function
Fully automated solubilization process and navigation for pod runs
Insert biodegradable pod to solubilize and dispense 500 mL of media
Proprietary mechanism for bottle uncapping and capping
In-unit UV-C light sterilization process
Designed to fit on standard lab benchtops
Consistency of your cell media product is critical to the success of your development. Our protocols for pod filling and solubilization ensure media with equivalent quality and solubilized ingredient specifications as off-the-shelf media. In a direct comparison of Krakatoa mixed media and off-the-shelf media (DMEM/F-12 supplemented with 10% FBS), both media showed equivalent levels of amino acids when compared to a control specification.
Producing media sustainably is our goal to cure the planet, but producing quality media is a necessity to cure patients. Krakatoa media performs comparably to off-the-shelf media when analyzing cell proliferation and population doubling. In a comparison of off-the-shelf DMEM/F-12 supplemented with 10% FBS vs Krakatoa DMEM/F12 supplemented with 10% FBS on MSCs, our data shows equivalent cell confluence % from Day 0 through Day 6.
Krakatoa was evaluated for its capability in mixing media supplemented with large proteins. In our study, we manufactured CMRL media, a complex media formulation supplemented with insulin and transferrin. Media was tested for protein content after filtration and it showed comparable levels to the specification, proving that Krakatoa is capable of mixing, dispensing, and filtering advanced media.
Manufacture Greener Therapies
Roughly 5.5M tons of plastic waste per year is produced by our industry, taking centuries to decompose. Krakatoa, the world’s first benchtop media maker, was built so that together we can create a cleaner future, reducing waste and greenhouse gas emissions.
Reduction in total greenhouse gas emissions per 500 mL of media produced.
Reduction in transportation greenhouse gas emissions per 500 mL of media produced.
Reduction in end-of-life greenhouse gas emissions per 500 mL of media produced.
Make Your Cell Culture Science Sustainable
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How It Works
Bottle and Pod Insertion
Solubilization and Sterilization
Every pod can be recycled through our recycling program— just seal the pod and place into the provided bags for shipping. We recommend no more than 10 per bag.
Changing the Way Cell Culture Media is Delivered
Can I purchase a Krakatoa™ directly from Stoic Bio?
Krakatoa is currently only available from our partner, Nucleus Biologics.
Does the Krakatoa™ device require cleaning?
Krakatoa runs an internal self-cleaning cycle automatically after every pod run. No input is required from the user to initiate this sequence. External cleaning may be performed periodically in accordance with the guidelines in the user manual.
What type of warranty does Krakatoa™ come with?
Krakatoa comes with a limited 12-month warranty.
Once I start a pod run, do I need to remain by the instrument during solubilization?
No. After inserting the pod and glass bottle, Krakatoa will run an automated pod run cycle and you do not need to be present for the solubilization stage of the pod run.
Resources and Downloads
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